Mouse Primary Aortic Endothelial Cells

Isolation and culture of endothelial cells from mouse aorta preparation of aorta rings 1.

Mouse primary aortic endothelial cells.

Enos knockout mice were kindly provided by dr. Wipe off skin with 70 isopropanol and fix mice on an operation table. Authors pedro molina sánchez 1 vicente andrés 2 affiliations 1 laboratory of molecular and genetic. Endothelial cells ecs are key blood vessel wall components that play critical roles in the regulation of many physiological processes including angiogenesis coagulation and vascular tone.

This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. The human umbilical vein endothelial cells were cultured by using cell passageway to further growth in the culture medium. Cut off tissue to open chest and abdomen and expose aorta. C57bl 6 mouse primary aortic endothelial cells can be used in assays of cell cell adhesion migration vascular tube formation or transendothelial resistance ter.

Method of primary cell culture with appropriate reagents. The mouse is a critical model for research however the isolation of primary ec from murine aorta is considered difficult. Standard biochemical procedures performed with endothelial cell cultures include rt pcr western blotting immunoprecipitation immunofluorescent staining immunofluorescent flow cytometry or generating cell derivatives for desired research applications. Paul huang cardiovascular.

Previously reported procedures for the isolation of ec have required magnetic beads or fluorescence activated cell sorting facs to purify the cells. Marek drab max planck institute for molecular cell biology and genetics dresden germany. The vascular endothelial cells play a significant role in many important cardiovascular disorders. Mouse aortic endothelial cells maec were isolated from the thoracic and abdominal aortas of various control and knockout mouse lines.

Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology. Euthanatize mice by co 2 asphyxiation followed by cervical dislocation. They produce endothelium derived substances regulating vasoconstriction and vessel growth. Isolation of mouse primary aortic endothelial cells by selection with specific antibodies methods mol biol.

Wild type c57bl 6 and p47 phox knockout mice were purchased from jackson laboratories bar harbor maine. Caveolin 1 cav1 knockout mice were kindly provided by dr. Mouse aortic endothelial cells maoec line the vessel wall of aorta and are constantly exposed to high hemodynamic forces. Isolation of mouse primary aortic endothelial cells by selection with specific antibodies springerlink.

Primary human aortic endothelial cells or haoecs acbri 375 were initiated from normal human descending aorta aortic arch to renal artery.