Studies on glucose 6 phosphate g6p phosphate translocator isoforms gpt1 and gpt2 reported the viability of arabidopsis arabidopsis thaliana gpt2 mutants whereas heterozygous gpt1 mutants exhibited a variety of defects during fertilization seed set indicating that gpt1 is essential for this process.
Mobitec m el.
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Eiche bianco teilmassiv front.
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Among other functions gpt1 was shown to be important for pollen and embryo sac development.
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Expansion microscopy exm enables super resolution fluorescence imaging of physically expanded biological samples with conventional microscopes.
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Fisher hmsir 647 a208 01 mobitec conjugated to goat anti rabbit igg f ab 2 sab3700946 sigma tetraspeck microspheres 0 1 µm t27279 thermo fisher.
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By combining exm with single molecule localization.
Nuclei were counterstained with 1 µg ml hoechst 33258 sigma aldrich munich germany.
Negative controls were incubated either omitting the primary antibody or blocking the primary antibody with the respective peptide.
Slides were covered using mobiglow mounting medium mobitec goettingen germany and appropriate cover slips.